Marshall University Music Department Presents the Marshall University Jazz Ensemble 12.0, Ed Bingham, director, with guest artist Jeff Wolfe, trumpet, assisted by Sean Parsons

https://mds.marshall.edu/music_perf/1447/thumbnail.jp

Saltwater based fractionation and valorisation of macroalgae

BACKGROUND: Macroalgae are gaining increasing interest as an important biomass feedstock. Yet when valorising marine bio�mass, the presence of salt can pose a substantial obstacle to the effectiveness of downstream biological and chemical processes, as well as the engineering infrastructure required. Accordingly, dewatering, washing and drying are often considered the first and crucial primary steps in processing marine biomass such macroalgae. The high costs of these processes can make further marine biorefinery commercialisation prohibitive. This investigation assesses simple pre-treatments for macroalgal biomass in saltwater, thereby reducing the freshwater footprint, and removing the need for an energy-intensive washing and drying stage. RESULTS: Using acid and basic catalysts, the carbohydrate and soluble protein components were fractionated into a soluble aqueous phase, for further fermentation and a solid phase suitable for hydrothermal liquefaction. The presence of saltwater was found to aid the fractionation process, solubilising more of the biomass. The use of H2SO4 produced more monosaccha�rides, whereas NaOH solubilised higher levels of biomass at lower temperatures. The aqueous phase was demonstrated to be suitable for biological processing with the salt tolerant yeast Metschnikowia pulcherrima, and the residual solids suitable for processing via hydrothermal liquefaction. CONCLUSION: By contrast with existing pre-treatment strategies, we demonstrate that an entirely salt-based biochemical con�version route is a potentially viable option. For the first time this work demonstrates that, rather than a hindrance, the presence of saltwater can be advantageous, and could provide an alternative, more cost-effective pathway to achieving a successful macroalgal-based biorefinery. © 2020 Society of Chemical Industr

Marshall University Music Department Presents Bluetrane, Faculty Jazz Ensemble, Featuring, Ed Bingham, saxophone, Mike Stroeher, trombone, Steve Hall, percussion Sean Parsons, piano, Martin Saunders, trumpet, Mark Zanter, guitar

https://mds.marshall.edu/music_perf/1498/thumbnail.jp

Low-Level Prenatal and Postnatal Blood Lead Exposure and Adrenocortical Responses to Acute Stress in Children

BACKGROUND: A few recent studies have demonstrated heightened hypothalamic–pituitary–adrenal (HPA) axis reactivity to acute stress in animals exposed to heavy metal contaminants, particularly lead. However, Pb-induced dysregulation of the HPA axis has not yet been studied in humans. OBJECTIVE: In this study, we examined children’s cortisol response to acute stress (the glucocorticoid product of HPA activation) in relation to low-level prenatal and postnatal Pb exposure. METHODS: Children’s prenatal blood Pb levels were determined from cord blood specimens, and postnatal lead levels were abstracted from pediatrician and state records. Children’s adrenocortical responses to an acute stressor were measured using assays of salivary cortisol before and after administration of a standard cold pressor task. RESULTS: Pb exposure was not associated with initial salivary cortisol levels. After an acute stressor, however, increasing prenatal and postnatal blood Pb levels were independently associated with significantly heightened salivary cortisol responses. CONCLUSIONS: Our results suggest that relatively low prenatal and postnatal blood lead levels— notably those below the 10 µg/dL blood lead level identified by the Centers for Disease Control and Prevention for public health purposes—can alter children’s adrenocortical responses to acute stress. The behavioral and health consequences of this Pb-induced HPA dysregulation in children have yet to be determined

Anatomy, morphology and evolution of the patella in squamate lizards and tuatara (Sphenodon punctatus)

The patella (kneecap) is the largest and best-known of the sesamoid bones, postulated to confer biomechanical advantages including increasing joint leverage and reinforcing the tendon against compression. It has evolved several times independently in amniotes, but despite apparently widespread occurrence in lizards, the patella remains poorly characterised in this group and is, as yet, completely undescribed in their nearest extant relative Sphenodon (Rhynchocephalia). Through radiography, osteological and fossil studies we examined patellar presence in diverse lizard and lepidosauromorph taxa, and using computed tomography, dissection and histology we investigated in greater depth the anatomy and morphology of the patella in 16 lizard species and 19 Sphenodon specimens. We have found the first unambiguous evidence of a mineralised patella in Sphenodon, which appears similar to the patella of lizards and shares several gross and microscopic anatomical features. Although there may be a common mature morphology, the squamate patella exhibits a great deal of variability in development (whether from a cartilage anlage or not, and in the number of mineralised centres) and composition (bone, mineralised cartilage or fibrotendinous tissue). Unlike in mammals and birds, the patella in certain lizards and Sphenodon appears to be a polymorphic trait. We have also explored the evolution of the patella through ancestral state reconstruction, finding that the patella is ancestral for lizards and possibly Lepidosauria as a whole. Clear evidence of the patella in rhynchocephalian or stem lepidosaurian fossil taxa would clarify the evolutionary origin(s) of the patella, but due to the small size of this bone and the opportunity for degradation or loss we could not definitively conclude presence or absence in the fossils examined. The pattern of evolution in lepidosaurs is unclear but our data suggest that the emergence of this sesamoid may be related to the evolution of secondary ossification centres and/or changes in knee joint conformation, where enhancement of extensor muscle leverage would be more beneficial.Sophie Regnault, Marc E. H. Jones, Andrew A. Pitsillides, John R. Hutchinso

The Burst Alert Telescope (BAT) on the Swift MIDEX Mission

The Burst Alert Telescope (BAT) is one of 3 instruments on the Swift MIDEX spacecraft to study gamma-ray bursts (GRBs). The BAT first detects the GRB and localizes the burst direction to an accuracy of 1-4 arcmin within 20 sec after the start of the event. The GRB trigger initiates an autonomous spacecraft slew to point the two narrow field-of-view (FOV) instruments at the burst location within 20-70 sec so to make follow-up x-ray and optical observations. The BAT is a wide-FOV, coded-aperture instrument with a CdZnTe detector plane. The detector plane is composed of 32,768 pieces of CdZnTe (4x4x2mm), and the coded-aperture mask is composed of approximately 52,000 pieces of lead (5x5x1mm) with a 1-m separation between mask and detector plane. The BAT operates over the 15-150 keV energy range with approximately 7 keV resolution, a sensitivity of approximately 10E-8 erg*cm^-2*s^-1, and a 1.4 sr (half-coded) FOV. We expect to detect >100 GRBs/yr for a 2-year mission. The BAT also performs an all-sky hard x-ray survey with a sensitivity of approximately 2 mCrab (systematic limit) and it serves as a hard x-ray transient monitor.Comment: 18 Pages, 12 Figures, To be published in Space Science Review

Listening forward: approaching marine biodiversity assessments using acoustic methods

© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Mooney, T. A., Di Iorio, L., Lammers, M., Lin, T., Nedelec, S. L., Parsons, M., Radford, C., Urban, E., & Stanley, J. Listening forward: approaching marine biodiversity assessments using acoustic methods. Royal Society Open Science, 7(8), (2020): 201287, doi:10.1098/rsos.201287.Ecosystems and the communities they support are changing at alarmingly rapid rates. Tracking species diversity is vital to managing these stressed habitats. Yet, quantifying and monitoring biodiversity is often challenging, especially in ocean habitats. Given that many animals make sounds, these cues travel efficiently under water, and emerging technologies are increasingly cost-effective, passive acoustics (a long-standing ocean observation method) is now a potential means of quantifying and monitoring marine biodiversity. Properly applying acoustics for biodiversity assessments is vital. Our goal here is to provide a timely consideration of emerging methods using passive acoustics to measure marine biodiversity. We provide a summary of the brief history of using passive acoustics to assess marine biodiversity and community structure, a critical assessment of the challenges faced, and outline recommended practices and considerations for acoustic biodiversity measurements. We focused on temperate and tropical seas, where much of the acoustic biodiversity work has been conducted. Overall, we suggest a cautious approach to applying current acoustic indices to assess marine biodiversity. Key needs are preliminary data and sampling sufficiently to capture the patterns and variability of a habitat. Yet with new analytical tools including source separation and supervised machine learning, there is substantial promise in marine acoustic diversity assessment methods.Funding for development of this article was provided by the collaboration of the Urban Coast Institute (Monmouth University, NJ, USA), the Program for the Human Environment (The Rockefeller University, New York, USA) and the Scientific Committee on Oceanic Research. Partial support was provided to T.A.M. from the National Science Foundation grant OCE-1536782

Survey of the quality of experimental design, statistical analysis and reporting of research using animals

For scientific, ethical and economic reasons, experiments involving animals should be appropriately designed, correctly analysed and transparently reported. This increases the scientific validity of the results, and maximises the knowledge gained from each experiment. A minimum amount of relevant information must be included in scientific publications to ensure that the methods and results of a study can be reviewed, analysed and repeated. Omitting essential information can raise scientific and ethical concerns. We report the findings of a systematic survey of reporting, experimental design and statistical analysis in published biomedical research using laboratory animals. Medline and EMBASE were searched for studies reporting research on live rats, mice and non-human primates carried out in UK and US publicly funded research establishments. Detailed information was collected from 271 publications, about the objective or hypothesis of the study, the number, sex, age and/or weight of animals used, and experimental and statistical methods. Only 59% of the studies stated the hypothesis or objective of the study and the number and characteristics of the animals used. Appropriate and efficient experimental design is a critical component of high-quality science. Most of the papers surveyed did not use randomisation (87%) or blinding (86%), to reduce bias in animal selection and outcome assessment. Only 70% of the publications that used statistical methods described their methods and presented the results with a measure of error or variability. This survey has identified a number of issues that need to be addressed in order to improve experimental design and reporting in publications describing research using animals. Scientific publication is a powerful and important source of information; the authors of scientific publications therefore have a responsibility to describe their methods and results comprehensively, accurately and transparently, and peer reviewers and journal editors share the responsibility to ensure that published studies fulfil these criteria

Detailed monitoring reveals the nature of submarine turbidity currents

Seafloor sediment flows, called turbidity currents, form the largest sediment accumulations, deepest canyons, and longest channels on Earth. It was once thought that turbidity currents were impractical to measure in action, especially due to their ability to damage sensors in their path, but direct monitoring since the mid 2010s has measured them in detail. In this Review, we summarise knowledge of turbidity currents gleaned from this direct monitoring. Monitoring identifies triggering mechanisms from dilute river-plumes, and shows how rapid sediment accumulation can precondition slope failure, but the final triggers can be delayed and subtle. Turbidity currents are consistently more frequent than predicted by past sequence stratigraphic models, including at sites >300 km from any coast. Faster (>~1.5 m s–1) flows are driven by a dense near-bed layer at their front, whereas slower flows are entirely dilute. This frontal layer sometimes erodes large (>2.5 km3) volumes of sediment, yet maintains a near-uniform speed, leading to a travelling wave model. Monitoring shows that flows sculpt canyons and channels through fast-moving knickpoints, and how deposits originate. Emerging technologies with reduced cost and risk can lead to widespread monitoring of turbidity currents, so their sediment and carbon fluxes can be compared with other major global transport processes

Personalized Pathway Enrichment Map of Putative Cancer Genes from Next Generation Sequencing Data

BACKGROUND: Pathway analysis of a set of genes represents an important area in large-scale omic data analysis. However, the application of traditional pathway enrichment methods to next-generation sequencing (NGS) data is prone to several potential biases, including genomic/genetic factors (e.g., the particular disease and gene length) and environmental factors (e.g., personal life-style and frequency and dosage of exposure to mutagens). Therefore, novel methods are urgently needed for these new data types, especially for individual-specific genome data. METHODOLOGY: In this study, we proposed a novel method for the pathway analysis of NGS mutation data by explicitly taking into account the gene-wise mutation rate. We estimated the gene-wise mutation rate based on the individual-specific background mutation rate along with the gene length. Taking the mutation rate as a weight for each gene, our weighted resampling strategy builds the null distribution for each pathway while matching the gene length patterns. The empirical P value obtained then provides an adjusted statistical evaluation. PRINCIPAL FINDINGS/CONCLUSIONS: We demonstrated our weighted resampling method to a lung adenocarcinomas dataset and a glioblastoma dataset, and compared it to other widely applied methods. By explicitly adjusting gene-length, the weighted resampling method performs as well as the standard methods for significant pathways with strong evidence. Importantly, our method could effectively reject many marginally significant pathways detected by standard methods, including several long-gene-based, cancer-unrelated pathways. We further demonstrated that by reducing such biases, pathway crosstalk for each individual and pathway co-mutation map across multiple individuals can be objectively explored and evaluated. This method performs pathway analysis in a sample-centered fashion, and provides an alternative way for accurate analysis of cancer-personalized genomes. It can be extended to other types of genomic data (genotyping and methylation) that have similar bias problems